โ Scribed by Vincan, Elizabeth
No coin nor oath required. For personal study only.
Activation of the canonical Wnt pathway leads to the transcriptional activation of a particular subset of downstream Wnt target genes. To track this localized cellular output in a living organism, reporter constructs can be designed containing multimerized consensus lymphoid enhancer binding factor (LEF)-1/T cell factor (TCF) transcription factor binding sites, generally referred to as TCF optimal promoter (TOP) sites. In Xenopus , several Wnt-responsive reporter systems have been designed containing a number of these TOP sites that, in combination with a minimal promoter, drive the expression of a reporter gene. Following transgenic integration in Xenopus embryos, a Wnt reporter tool reveals the spatiotemporal delineation of endogenous Wnt pathway activities throughout development. Assumed to be a general readout of the Wnt pathway, such reporters can assist in elucidating unknown functional implications in developing Xenopus embryos.
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