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[Methods in Molecular Biology] Protein Electrophoresis Volume 869 || Silver Staining Techniques of Polyacrylamide Gels

✍ Scribed by Kurien, Biji T.; Scofield, R. Hal

Book ID
111692111
Publisher
Humana Press
Year
2012
Weight
203 KB
Category
Article
ISBN
1617798215

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✍ Kurien, Biji T.; Scofield, R. Hal πŸ“‚ Article πŸ“… 2012 πŸ› Humana Press βš– 216 KB

Usually proteins are separated by polyacrylamide gel electrophoresis (PAGE) in the presence of a detergent and under (heat-) denaturing and (non- or) reducing conditions. The most commonly used detergent is sodium dodecyl sulfate (SDS). The major function of SDS is to shield the respective charge of