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[Methods in Molecular Biology] LC-MS in Drug Analysis Volume 902 || Simultaneous Determination of Cyclosporine, Sirolimus, and Tacrolimus in Whole Blood Using Liquid Chromatography–Tandem Mass Spectrometry

✍ Scribed by Langman, Loralie J.; Snozek, Christine L. H.

Book ID: 111939049
Publisher: Humana Press
Year: 2012
Weight: 241 KB
Category: Article
ISBN: 1617799343
DOI: 10.1007/978-1-61779-934-1_15

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## Abstract A LC‐MS/MS method was developed and validated for the determination of cyclosporine A (CsA) and its three phase 1 metabolites AM1, AM9, and AM4N in whole blood and lymphocytes isolated on the Histopaque gradient. 200 μL of whole blood was precipitated with 10 mol/L zinc sulfate in aceto