[Methods in Molecular Biology] Heart Proteomics Volume 1005 || Characterization of Zebrafish Cardiac Proteome Using Online pH Gradient SCX–RP HPLC–MS/MS Platform

Characterization of the adult zebrafish

Characterization of the adult zebrafish cardiac proteome using online pH gradient strong cation exchange-RP 2D LC coupled with ESI MS/MS

✍ Jiang Zhang; Kevin A. Lanham; Richard E. Peterson; Warren Heideman; Lingjun Li 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 344 KB

## Abstract 2D HPLC separations by coupling strong cation exchange (SCX) and RP fractionation have been widely used in large‐scale proteomic studies. Traditionally this method is performed by salt gradient SCX separation followed by RP and MS/MS analysis. The salt gradient SCX method has been known