[Methods in Molecular Biology] Forensic Microscopy for Skeletal Tissues Volume 915 || Preparation of Mineralized Tissue for Light Microscopy

✍ Scribed by Bell, Lynne S.

• Book ID: 120364064
• Publisher: Humana Press
• Year: 2012
• Weight: 457 KB
• Category: Article
• ISBN: 1617799777
• DOI: 10.1007/978-1-61779-977-8_3

✦ Synopsis

Production of stained tissue sections for examination by light microscopy is a step-wise process which begins with preservation of tissue ( fi xation), then dehydration and clearing of the tissue, and fi nally impregnation with wax (processing). Mineralized tissues such as bone and teeth are subject to a further step (decalci fi cation). Thin sections are then attached to a glass slide for staining and light microscopy.In the UK, it is usual for a preliminary diagnosis to be made using hematoxylin and eosin (H&E) staining. A de fi nitive diagnosis may need further investigation with immunocytochemistry. All of these procedures must allow morphology and tissue structure to remain in tact, as any pathology present must not be compromised.