[Methods in Molecular Biology] Cell-Free Protein Production Volume 607 || Cell-Free Protein Synthesis Systems with Extracts from Cultured Human Cells

✍ Scribed by Endo, Yaeta; Takai, Kazuyuki; Ueda, Takuya

• Book ID: 118125447
• Publisher: Humana Press
• Year: 2009
• Tongue: English
• Weight: 789 KB
• Edition: 2010
• Category: Article
• ISBN: 160327331X
• DOI: 10.1007/978-1-60327-331-2_5

✦ Synopsis

1. Cell-free Protein Preparation Through Prokaryotic Transcription-translation Methods / Takanori Kigawa -- 2. Pure Technology / Yoshihiro Shimizu And Takuya Ueda -- 3. The Cell-free Protein Synthesis System From Wheat Germ / Kazuyuki Takai And Yaeta Endo -- 4. A Cell-free Protein Synthesis System From Insect Cells / Toru Ezure, Takashi Suzuki, Masamitsu Shikata, Masaaki Ito, And Eiji Ando -- 5. Cell-free Protein Synthesis Systems With Extracts From Cultured Human Cells / Satoshi Mikami, Tominari Kobayashi, And Hiroaki Imataka -- 6. Analysis Of Protein Functions Through A Bacterial Cell-free Protein Expression System / Takanori Kigawa. 7. Cell-free Based Protein Microarray Technology Using Agarose/dna Microplate / Tatsuya Sawasaki And Yaeta Endo -- 8. An Efficient Approach To The Production Of Vaccines Against The Malaria Parasite / Takafumi Tsuboi, Satoru Takeo, Tatsuya Sawasaki, Motomi Torii, And Yaeta Endo -- 9. Protein Engineering Accelerated By Cell-free Technology / Takuya Kanno And Yuzuru Tozawa -- 10. Cell-free Protein Production System With The E. Coli Crude Extract For Determination Of Protein Folds / Takanori Kigawa -- 11. Nmr Assignment Method For Amide Signals With Cell-free Protein Synthesis System / Toshiyuki Kohno. 12. Cell-free Protein Synthesis Technology In Nmr High-throughput Structure Determination / Shin-ichi Makino, Michael A. Goren, Brian G. Fox, And John L. Markley -- 13. Cell-free Protein Synthesis For Structure Determination By X-ray Crystallography / Miki Watanabe, Ken-ichi Miyazono, Masaru Tanokura, Tatsuya Sawasaki, Yaeta Endo, And Ichizo Kobayashi -- 14. Production Of Multi-subunit Complexes On Liposome Through An E. Coli Cell-free Expression System / Yutetsu Kuruma, Toshiharu Suzuki, And Takuya Ueda -- 15. Synthesis Of A Hetero Subunit Rna Modification Enzyme By The Wheat Germ Cell-free Translation System / Hiroyuki Hori. 16. Strategies For The Cell-free Expression Of Membrane Proteins / Sina Reckel, Solmaz Sobhanifar, Florian Durst, Frank Löhr, Vladimir A. Shirokov, Volker Dötsch, And Frank Bernhard -- 17. Production Of Membrane Proteins Through The Wheat-germ Cell-free Technology / Akira Nozawa, Hideaki Nanamiya, And Yuzuru Tozawa -- 18. Ribosome Display With The Pure Technology / Takashi Kanamori, Hiroyuki Ohashi, And Takuya Ueda -- 19. Incorporation Of 3-azidotyrosine Into Proteins Through Engineering Yeast Tyrosyl-trna Synthetase And Its Application To Site-selective Protein Modification / Takashi Yokogawa, Satoshi Ohno, And Kazuya Nishikawa. 20. Synthesis Of Functional Proteins Within Liposomes / Takeshi Sunami, Tomoaki Matsuura, Hiroaki Suzuki, And Tetsuya Yomo Edited By Yaeta Endo, Kazuyuki Takai, Takuya Ueda. Includes Bibliograpical References And Index.