[Methods in Molecular Biology] Astrocytes Volume 814 || Induction of Nitric Oxide Synthase-2 Expression and Measurement of Nitric Oxide Production in Enriched Primary Cortical Astrocyte Cultures

Functional expression of constitutive ni

Functional expression of constitutive nitric oxide synthases regulated by voltage-gated Na+ and Ca2+ channels in cultured human astrocytes

✍ Michiko Oka; Miyuki Wada; Akira Yamamoto; Yoshinori Itoh; Takuya Fujita 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 437 KB

## Abstract We report the functional characterization of constitutive nitric oxide synthase(s) (NOS) such as neuronal and endothelial NOS in cultured human astrocytes. Exposure of cultured human astrocytes to 1 μM veratridine or 50 mM KCl produced a pronounced increase in a calmodulin‐dependent NOS