Methods in Methane Metabolism, Part A

✍ Scribed by Amy C. Rosenzweig and Stephen W. Ragsdale (Eds.)

• Publisher: Academic Press
• Year: 2011
• Tongue: English
• Leaves: 401
• Series: Methods in Enzymology 494
• Edition: 1
• Category: Library

✦ Synopsis

Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field.

Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field

✦ Table of Contents

Content:
Title Page
Page ii

Copyright
Page iv

Contributors
Pages xi-xiv

Preface
Pages xv-xvi
Amy C. Rosenzweig, Stephen W. Ragsdale

Contents of previous volume
Pages xvii-xlv

Chapter one - Techniques for Cultivating Methanogens
Pages 1-22
Ralph S. Wolfe

Chapter two - Genetic Methods for Methanosarcina Species
Pages 23-42
Nicole Buan, Gargi Kulkarni, William Metcalf

Chapter three - Genetic Systems for Hydrogenotrophic Methanogens
Pages 43-73
Felipe B. Sarmiento, John A. Leigh, William B. Whitman

Chapter four - Molecular Tools for Investigating ANME Community Structure and Function
Pages 75-90
Steven J. Hallam, Antoine P. PagГ©, Lea Constan, Young C. Song, Angela D. Norbeck, Heather Brewer, Ljiljana Pasa-Tolic

Chapter five - Studying Gene Regulation in Methanogenic Archaea
Pages 91-110
Michael Rother, Christian Sattler, Tilmann Stock

Chapter six - Growth of Methanogens Under Defined Hydrogen Conditions
Pages 111-118
John A. Leigh

Chapter seven - Preparation of [Fe]-Hydrogenase from Methanogenic Archaea
Pages 119-137
Seigo Shima, Michael Schick, Haruka Tamura

Chapter eight - Assay of Methylotrophic Methyltransferases from Methanogenic Archaea
Pages 139-158
Donald J. Ferguson Jr.,, David G. Longstaff, Joseph A. Krzycki

Chapter nine - Methyl-Coenzyme M Reductase from Methanothermobacter marburgensis
Pages 159-187
Evert C. Duin, Divya Prakash, Charlene Brungess

Chapter ten - Methods for Analysis of Acetyl-CoA Synthase: Applications to Bacterial and Archaeal Systems
Pages 189-217
David A. Grahame

Chapter eleven - Acetate Kinase and Phosphotransacetylase
Pages 219-231
James G. Ferry

Chapter twelve - Sodium Ion Translocation and ATP Synthesis in Methanogens
Pages 233-255
Katharina Schlegel, Volker MГјller

Chapter thirteen - Proton Translocation in Methanogens
Pages 257-280
Cornelia Welte, Uwe Deppenmeier

Chapter fourteen - Measuring Isotope Fractionation by Autotrophic Microorganisms and Enzymes
Pages 281-299
Kathleen M. Scott, Gordon Fox, Peter R. Girguis

Chapter fifteen - 2-Oxoacid Metabolism in Methanogenic CoM and CoB Biosynthesis
Pages 301-326
David E. Graham

Chapter sixteen - Biomethanation and Its Potential
Pages 327-351
Irini Angelidaki, Dimitar Karakashev, Damien J. Batstone, Caroline M. Plugge, Alfons J.M. Stams

Author Index
Pages 353-365

Subject Index
Pages 367-373