[Methods in Enzymology] Neoglycoconjugates, Part B: Biomedical Applications Volume 247 || [15] Affinity chromatography of oligosaccharides on Psathyrella velutina lectin column
✍ Scribed by Kobata, Akira
- Book ID
- 120258636
- Publisher
- Elsevier
- Year
- 1994
- Tongue
- English
- Weight
- 464 KB
- Edition
- 1
- Category
- Article
- ISBN
- 012182148X
- ISSN
- 0076-6879
No coin nor oath required. For personal study only.
✦ Synopsis
Neoglycoconjugates are not only useful for the basic understanding of protein-carbohydrate interactions, but they have many practical applications as well. They are powerful reagents in many cell biology studies and excellent tools for the isolation and characterization of animal and plant lectins, separation of cells, as well as for the targeting of drugs, artificial vaccines, and diagnostic reagents. Volume 247 and its companion Volume 242 contain many practical methods on how to prepare and use neoglycoconjugates. Volume 242 deals with synthesis and 247 with biomedical applications.
C. Harry Arsenis
This volume in the Methods in Enzymology series deals with the biomedical applications of this group of neoglycoconjugates. There are several chapters dealing with the synthesis of conjugates attached to lipids, proteins and insoluble carbohydrates. The purpose is to describe the applications of different neoglycoconjugates bound to different materials of lipid, protein, or carbohydrate origin. The conjugated moieties have a natural origin or have been synthetically made. The objectives of the book are particularly worthy in view of the recent interest in the use of natural products in medicine and industry. The specific objective of the book is to describe the applications of the chemically synthesized or natural glycoconjugates. The book meets the authors' objectives. The book is written for chemists and other research scientists who are in search of synthetic or natural matrices for the testing of different ligands in medicine or industry. The book will additionally be interesting to diversified scientists in various industries who are looking for an effective Bed. The methodologies described provide accurate procedures for the application of chemomimetics for laboratory or industrial use. The book is written for the advanced student or researcher specialist in search of a matrix-ligand combination suitable for his or her system of study. The neoglycoconjugates may find applications in a variety of industries not related to medicine or drug development. The book is adequately illustrated. The references are quite current (latest 1993) and pertinent for the subject. Cells communicate with their environment by synthesizing and using as sensing devices a variety of carbohydrate-basedmolecules. These moieties are attached to various fixed or insoluble structures, such as for example, membranes. Consequently, the chemical synthesis or isolation in pure form of these conjugates is a valuable system for research and development in both medicine and industry. The research and exploration of these carbohydrate conjugates necessitates the attachment of these ligands onto some structure resembling their natural carrier, a protein, a lipid, or another insoluble carbohydrate. Consequently, the methodology on the applications of these conjugates is extremely essential. The research and application of the findings demand that these products are immobilized or attached to various matrices. The book provides a useful compilation of the methodologies for testing and using these materials (the conjugates). The dedication of a separate volume on the application of the neoglycoconjugates seems to be a good idea.