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[Methods in Enzymology] Laboratory Methods in Enzymology: Protein Part B Volume 539 || UV Cross-Linking of Interacting RNA and Protein in Cultured Cells

✍ Scribed by Sei, Emi

Book ID: 126009393
Publisher: Elsevier
Year: 2014
Tongue: English
Weight: 920 KB
Edition: 1
Category: Article
ISBN: 0124201202
ISSN: 0076-6879
DOI: 10.1016/B978-0-12-420120-0.00004-9

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✦ Synopsis

RNA-protein interactions play indispensable roles in the regulation of cellular functions. Biochemical characterization of these complexes is often done by immunoprecipitation (IP) of RNA-binding proteins (RBPs) followed by identification of co-immunoprecipitated RNAs. This protocol couples ultraviolet (UV) irradiation with IP to determine whether a specific protein interacts directly with a specific RNA in living cells.

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