[Methods in Enzymology] Guide to Protein Purification Volume 182 || [23] Precipitation of proteins with polyethylene glycol

✍ Scribed by Ingham, Kenneth C.

• Book ID: 120518414
• Publisher: Elsevier
• Year: 1990
• Tongue: English
• Weight: 322 KB
• Edition: 1
• Category: Article
• ISBN: 0121820831
• ISSN: 0076-6879
• DOI: 10.1016/0076-6879(90)82025-W

✦ Synopsis

Polyethylene glycol is a nondenaturing water-soluble polymer whose ability to precipitate protein from aqueous solution can be qualitatively understood in terms of an excluded volume mechanism. The increment in PEG concentration required to effect a given reduction in solubility is unique for a given protein-polymer pair, being insensitive to solution conditions and primarily dependent on the size of the protein and polymer. Selective manipulation of the solubility of specific proteins through control of their state of association or ligand environment can potentially remove some of the empiricism otherwise involved in fractional precipitation. Adequate methods for removing the polymer are available.