[Methods in Enzymology] Fluorescence Spectroscopy Volume 450 || Chapter 12 Using Fluorophore-Labeled Oligonucleotides to Measure Affinities of Protein–DNA Interactions

✍ Scribed by Anderson, Brian J.

• Book ID: 120368564
• Publisher: Elsevier
• Year: 2008
• Tongue: English
• Weight: 662 KB
• Edition: 1
• Category: Article
• ISBN: 0123745861
• ISSN: 0076-6879
• DOI: 10.1016/S0076-6879(08)03412-5

✦ Synopsis

Fluorescence spectroscopy is a type of electromagnetic spectroscopy, using a beam of light, which analyzes fluorescence from a sample. Given its extremely high sensitivity and selectivity, it is an important investigational tool in many areas including material sciences, analytical sciences, and across a broad range of chemical, biochemical and medical research. It has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems. The pharmaceutical industry uses it heavily and it has become a dominating technique in biochemistry and molecular genetics.

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