Methods in Endothelial Cell Biology

✍ Scribed by Nancy Laurens, Victor W. M. van Hinsbergh (auth.), Prof.Dr. Hellmut G. Augustin (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 2004
• Tongue: English
• Leaves: 380
• Series: Springer Lab Manuals
• Edition: 1
• Category: Library

✦ Synopsis

Endothelial cell biology has developed into a vibrant discipline and has become a critical instrument to study several disease processes on the cellular and molecular level. It is now widely recognized that dysfunctions of normal endothelial cell homeostasis are involved in some of the most important human diseases, including ischemic heart diseases, hypertension, atherosclerosis, tumors, diabetes, arthritis, and inflammation. Further, the increasing importance and recognition of the field of vascular biology in general requires in vitro and in vivo techniques in order to address the complex questions.

Methods in Endothelial Cell Biology is a comprehensive practical "how-to"-guide summarizing the most relevant established techniques as well as a number of new emerging techniques. Easy-to-follow reliable protocols provide a useful lab bench resource for the experienced researcher and newcomer to the field.

✦ Table of Contents

Front Matter....Pages I-XX
Front Matter....Pages 1-1
Isolation,Purification and Culture of Human Micro- and Macrovascular Endothelial Cells....Pages 3-13
Isolation of Mouse Cardiac Microvascular Endothelial Cells....Pages 15-22
Isolation,Culture, and Differentiation of Circulating Endothelial Precursor Cells....Pages 23-32
Embryonic Stem Cell-Derived Endothelial Cells....Pages 33-45
Detection, Isolation and Culture of Lymphatic Endothelial Cells....Pages 47-54
Three-Dimensional Spheroid Culture of Endothelial Cells....Pages 55-62
Immortalization of Endothelial Cells....Pages 63-72
Viral Gene Transfer Into Endothelial Cells....Pages 73-82
Front Matter....Pages 83-83
Leukocyte Adhesion to Endothelium in Vitro Under Shear Stress....Pages 85-91
Application of Biomechanical Forces on Endothelial Cells....Pages 93-101
Endothelial Cell Permeability Assays in Culture....Pages 103-113
Three-Dimensional in Vitro Angiogenesis Assays....Pages 115-123
Rat Aortic Ring Assay of Angiogenesis....Pages 125-144
Endothelial Cell Chemotaxis and Chemokinesis Assays....Pages 145-156
FACS Analysis of Endothelial Cells....Pages 157-165
Application of the RNA Interference (RNAi) Technology to Angiogenesis Research....Pages 167-176
Front Matter....Pages 177-177
Murine Bone Marrow Transplantation Models that Enable the Study of EPC Recruitment....Pages 179-185
Balloon Denudation of Blood Vessels....Pages 187-195
Peripheral Hind Limb Ischemia Models....Pages 197-206
In Vivo Matrigel Angiogenesis Assays....Pages 207-222
Front Matter....Pages 177-177
Analysis of Vascular Permeability in Vivo....Pages 223-232
Validating the Manipulation of Endothelial Cell Signaling in Vivo....Pages 233-245
Chick Chorioallantoic Membrane Assay: Growth Factor and Tumor-induced Angiogenesis and Lymphangiogenesis....Pages 247-261
Corneal Angiogenesis Assay....Pages 263-272
Front Matter....Pages 273-273
Basic Blood Vessel Immunohistochemistry....Pages 275-286
Double-Staining Immunohistochemistry of Blood Vessels....Pages 287-299
In Situ Hybridization Analysis of Vascular Endothelium....Pages 301-312
High-Resolution in Situ Confocal Analysis of Endothelial Cells....Pages 313-323
Whole Mount Analysis of the Embryonic Vasculature....Pages 325-336
Methods for Visualizing Intact Blood Vessels Using Immunofluorescent Localization of PECAM (CD31) and α-Smooth Muscle Actin....Pages 337-345
Intravital Multi-fluorescence Microscopy....Pages 347-355
Corrosion Cast Analysis of Blood Vessels....Pages 357-369
Back Matter....Pages 371-383

✦ Subjects

Cell Biology; Human Physiology; Molecular Medicine; Angiology