Methods in Cell Biology, 42B : Flow Cytometry

✍ Scribed by Zbigniew Darzynkiewicz; John Paul Robinson; Harry A Crissman

• Publisher: Elsevier, Academic Press
• Year: 1994
• Tongue: English
• Leaves: 701
• Series: Methods in Cell Biology 42
• Edition: 2nd
• Category: Library

✦ Synopsis

From the Reviews of the First Edition:

''This is a good reference manual for multi-user facility faced with a wide variety of biological applications.''
- CYTOMETRY

''Flow Cytometry includes an impressive array of methods applicable to chromosome analysis, plant biology, marine biology, fluorescence, insitu hybridization, and others. It succeeds in providing the reader with good insight into the power of the technology throughout biology.''
- KENNETH A. AULT, MAINE CYTOMETRY RESEARCH INSTITUTE, MAINE MEDICAL CENTER, IN CANCER CELLS

Flow Cytometry, Second Edition provides a complete and comprehensive two-volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who use flow Cytometry to separate and analyze cells.



Comprehensive methodological coverage in unique style
In depth treatment of procedures
Description of each procedure's:

Theoretical foundations

Critical aspects

Possible pitfalls
Written by authors with extensive experience who:

Developed or modified the technique

Describe their experience with different instruments and applications to different cell systems

Are the Who's Who in Flow Cytometry
10 methods cover assessment of apoptosis and other modes of cell death
Practical, handbook-style presentation works in lab or classroom
Printed on acid-free paper
* Color plates

✦ Table of Contents

Content: Front Cover; Methods in Cell Biology: Flow Cytometry; Copyright Page; Content; Contents of Volume 41; Contributors; Preface to the Second Edition; Preface to the First Edition; Chapter 1. A Flow Cytometric Technique for Detection of DNA Repair in Mammalian Cells; Chapter 2. Rapid Determination of Cellular Resistance-Related Drug Efflux in Tumor Cells; Chapter 3. Glutathione and Cellular Resistance to Anti-Cancer Drugs; Chapter 4. Assay of Cell Resistance to Ara-C; Chapter 5. Detection of mRNA Species by Flow Cytometry. Chapter 6. Primed in Situ Labeling (PRINS) and Fluorescence in Situ Hybridization (FISH)Chapter 7. Molecular Phenotyping by Flow Cytometry; Chapter 8. Isolation and Analysis of Somatic Cell Mutants with Defects in Endocytic Traffic; Chapter 9. Measurement of Micronuclei by Flow Cytometry; Chapter 10. Sperm Chromatin Structure Assay: DNA Denaturability; Chapter 11. Fine-Needle Cytopuncture and Flow Cytometry in the Characterization of Human Tumors; Chapter 12. Functional N K Assays Using.
Abstract: From the Reviews of the First Edition:. ''This is a good reference manual for multi-user facility faced with a wide variety of biological applications.''. - CYTOMETRY. ''Flow Cytometry includes an impressive array of methods applicable to chromosome analysis, plant biology, marine biology, fluorescence, insitu hybridization, and others. It succeeds in providing the reader with good insight into the power of the technology throughout biology.''. - KENNETH A. AULT, MAINE CYTOMETRY RESEARCH INSTITUTE, MAINE MEDICAL CENTER, IN CANCER CELLS. Flow Cytometry, Second Edition provides a