Methods for the separation and identification of ribonucleotides on DEAE-cellulose

✍ Scribed by Youcef M. Rustum; Herbert S. Schwartz

• Book ID: 102983141
• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 578 KB
• Volume: 53
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(73)90088-2

✦ Synopsis

Methods have been developed for the separation and identification of ribonucleotides.

These methods include single gradient column chromatography with DEAE-cellulose type DE52 to separate putine and pyrimidine ribonucleotides, and PEI-cellulose to separate purine ribonucleotides. Sequential thin layer chromatography with PEI-cellulose may be adapted for the identification and confirmation of the separated components. Application of the methods is demonstrated with a rat liver extract.