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Methods for measurement of antibody/antigen affinity based on ELISA and RIA

✍ Scribed by Michel E. Goldberg; Lisa Djavadi-Ohaniance

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
589 KB
Volume
5
Category
Article
ISSN
0952-7915

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✦ Synopsis

Various

enzyme-linked immunosorbent assay or radioimmunoassay methods are currently used to quantify the antibody-antigen interaction.

Only those based on indirect competition -enzyme-linked immunosorbent assay or radioimmunoassay-can provide the real thermodynamic affinity of the antibody for its antigen. They can be applied to a variety of experimental situations, some of which are reviewed here.

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Traditional ELISA methods for antibody affinity determination fail to reveal the presence of low affinity antibodies in antisera: an alternative approach
✍ Sergei A. Bobrovnik; Miroslava Demchenko; Sergei Komisarenko; Fred Stevens πŸ“‚ Article πŸ“… 2009 πŸ› John Wiley and Sons 🌐 English βš– 354 KB

## Abstract Traditionally used methods of antibody affinity determination either by ELISA or by the surface plasmon resonance technique do not allow detection of the presence of low‐affinity antibodies in samples of high‐affinity antibodies. In this paper we demonstrate the possibility to reveal th