Methods for improving the yield and quality of metaphase preparations for FISH probing of human lymphocyte chromosomes

✍ Scribed by A.F. McFee; A.M. Sayer; S.I. Salomaa; C. Lindholm; L.G. Littlefield

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 285 KB
• Volume: 29
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/(sici)1098-2280(1997)29:1<98::aid-em13>3.0.co;2-c

✦ Synopsis

Procedures are described for the in vitro culture of well-spread metaphase preparations with little human lymphocytes, which have been concentrated background material to interfere with fluorescence by density gradient centrifugation, and for a modi-in situ hybridization (FISH) probe procedures. The fied slide-making technique for the fixed cells. The FISH reagents and microscope scanning time remethod yields improved percentages of mitotic cells quired are minimized by concentrating cells in a which are largely synchronized at harvest. Con-defined area of the slide.