Methodological improvement of the protein phosphatase inhibition assay for the detection of okadaic acid in mussels

✍ Scribed by R. Della Loggia; S. Sosa; A. Tubaro

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 57 KB
• Volume: 7
• Category: Article
• ISSN: 1056-9014
• DOI: 10.1002/1522-7189(199911/12)7:6<387::aid-nt87>3.0.co;2-n

✦ Synopsis

A simpli®ed procedure for the enzyme inhibition assay to measure okadaic acid and DTX-1 in mussels, based on the use of a commercially available enzyme preparation, is presented. The detection limit is 10 ng of toxin per g of digestive glands. Using Certi®ed Reference Material (MUS-2), high accuracy and good precision is demonstrated for contamination levels higher than 32 ng g À1 . Twenty samples can be processed in about 9 h by one operator, at the cost of US$ 10 per sample. Some possibilities for further enhancing the sensitivity and reducing the processing time are discussed and a monitoring example is presented.