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Method to purify mitochondrial DNA directly from yeast total DNA

✍ Scribed by Jingwen Zhou; Liming Liu; Jian Chen


Book ID
113855457
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
206 KB
Volume
64
Category
Article
ISSN
0147-619X

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Double-mutant cells of the budding yeast Saccharomyces cerevisiae harboring the gcs1-1 and sed1-1 mutations are conditionally defective (cold-sensitive) only for reentry into the mitotic cycle from stationary phase. If already proliferating at the permissive temperature (29 degrees C), these reentry