Consecutive enzymatic reactions on analytes affinity-bound to immobilized metal ion beads with subsequent direct analysis of the products by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry have been used for detecting protein synthesis errors occuring at the N-terminus.
✦ LIBER ✦
Method of Visualization of Matrix-Bound Proteins : Leucine Aminopeptidase Bound to Sepharose and Sephadex Beads
✍ Scribed by Jürgen LASCH; Martin IWIG; Horst HANSON
- Book ID
- 115113159
- Publisher
- John Wiley and Sons
- Year
- 1972
- Tongue
- English
- Weight
- 598 KB
- Volume
- 27
- Category
- Article
- ISSN
- 1432-1327
No coin nor oath required. For personal study only.
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## Abstract Double‐stranded DNA can be readily adsorbed on mica or directly on carbon coated grids from the surface of solutions containing ethidium bromide, actinomine, or propidium diiodide. The DNA molecules are unfolded, well separated, and show a length distribution similar to molecules prepar