✦ LIBER ✦

Method of imaging low density lipoproteins by atomic force microscopy

✍ Scribed by Julie A. Chouinard; Abdelouahed Khalil; Patrick Vermette

Publisher: John Wiley and Sons
Year: 2007
Tongue: English
Weight: 145 KB
Volume: 70
Category: Article
ISSN: 1059-910X
DOI: 10.1002/jemt.20492

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

This short paper reports a simple method to image low density lipoproteins (LDL) using atomic force microscopy (AFM). This instrument allows imaging of biological samples in liquid and presents the advantage of needing no sample preparation such as staining or fixation that may affect their general structure. Dimensions (diameter and height) of individual LDL particles were successfully measured. AFM imaging revealed that LDL have a quasi‐spherical structure on the x and y axis with an oblate spheroid structure in the z axis (i.e., height). LDLs were found to have an average diameter of 23 ± 3 nm. The obtained mean height was 10 ± 2 nm. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.

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