Method of detecting antibodies produced by hybridomas to cell nuclear endonucleases
โ Scribed by N. N. Khodarev; V. V. Volgina; D. V. Korogodin; I. I. Votrin
- Publisher
- Springer US
- Year
- 1987
- Tongue
- English
- Weight
- 266 KB
- Volume
- 103
- Category
- Article
- ISSN
- 0007-4888
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๐ SIMILAR VOLUMES
Antibody-producing hybridoma cells specifically bind to microgram quantities of antigen molecules adsorbed onto the surface of plastic microtiter plates. The binding of hybridoma cells to nonantigen is optimally below 5 % , similar binding of non-antibody-producing cells is 4-7 % , compared to the b
## Several analytical techniques were applied to obtain information about the stability of expression, yield and integrity of monoclonal antibodies (Mabs) produced by hybridoma cells in homogeneous continuous perfusion culture systems. An isotype-specific spot enzyme-linked immunosorbent assay (sp