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Method for the determination of pregnenolone in biological samples using gas-phase chromatography

✍ Scribed by W.P. Pulsinelli; R.K. Tcholakian; K.B. Eik-Nes


Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
510 KB
Volume
49
Category
Article
ISSN
0003-2697

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An alternative method for the determination of [ 'SN]ammonia enrichment in biological fluids was developed. It is based on the use of glutamate dehydrogenase of bovine liver (EC 1.4.1.2.) with 2-oxopentanoic acid as substrate, to convert the ammonia present in the sample into norvaline, the enrichme