Method for synthesis of 2-azido-N(6)-m-tritiobenzylaminopurine, a photoaffinity label for cytokinin-binding proteins in plants

✍ Scribed by Geoffrey Cooper; James Bourell; Miroslav Kaminek; J. Eugene Fox

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: French
• Weight: 190 KB
• Volume: 25
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580250906

✦ Synopsis

A method for tritiation of ZazideN(6)-buuyladenine (AZBA), a photoaffinity analog of the plant hormone N(6)-benzyladenine (BA), WYM developed. The synthetic acquence involves condensation of 2,6dichloropurine with m-iodobenrylamine to yield 2chloro-N(6)-m-iodobenzylade~e, followed by selective hydrodeiodination with tritium gas over palladium catalyst in pyridine solution to give 2-chlo~tN(6)-m-tritiobenzyl.dcnine. conversion of thin compound to AZBA enables preparation of the photosfZinity label compound with specific activity high enough to permit detection of cytokinin-binding proteins existing at low concentrations in plant tiaauea.