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Method for sequential staining of GTL-banded metaphases with fluorescent-labeled chromosome-specific paint probes

✍ Scribed by Jalal, Syed M. ;Law, Mark E. ;Christensen, Eric R. ;Spurbeck, Jack L. ;Dewald, Gordon W.


Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
649 KB
Volume
46
Category
Article
ISSN
0148-7299

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✦ Synopsis


We describe a method for use of fluorescent-labeled whole chromosome-specific paint probes on GTL-banded metaphases to utilize the combined potential of these techniques for defining chromosome abnormalities. The efficacy of this method was tested on 6 cases involving Merent chromosome abnormalities and various tissues, including blood, amniotic fluid, skin fibroblasts, and bone marrow.