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Method for Sequencing Synthetic Oligodeoxynucleotide Phosphorothioates

✍ Scribed by J.Y. Tang; A.M. Roskey; S. Agrawal

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 305 KB
Volume: 212
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1993.1302

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✦ Synopsis

Sequencing of oligodeoxynucleotide phosphorothioate by a modified Sanger method of sequencing is described. The procedure involves ligation of synthetic oligodeoxynucleotide phosphorothioate to an oligodeoxynucleotide, referred to here as "helper oligonucleotide." The helper oligonucleotide has a region which is complementary to (\mathrm{T} 7) primer. By using DNA polymerase and nucleoside triphosphate mixture, 5 '-labeled (\mathrm{T7}) primer is extended onto ligated oligodeoxynucleotide phosphorothioate, which is then analyzed on gel electrophoresis. 1993 Academic Press, Inc.

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