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Method for rapid isolation of free aminoacyl-transfer RNA

โœ Scribed by C. Judes; M. Jacob

Book ID
102626433
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
297 KB
Volume
50
Category
Article
ISSN
0003-2697

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A Continuous Spectrophotometric Assay for the Aminoacylation of Transfer RNA by Alanyl-Transfer RNA Synthetase
โœ M.X. Wu; K.A.W. Hill ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 272 KB

A continuous spectrophotometric assay is described for the aminoacylation reaction catalyzed by Escherichia coli alanyl-transfer RNA synthetase. The assay is based on coupling the alanyl-tRNA synthetase-dependent formation of AMP to the lactate dehydrogenase oxidation of NADH. Oxidation of NADH, as