✦ LIBER ✦
Method for identification of intracellular free flavin species in the photosensitive fungus Neurospora crassa
✍ Scribed by Lutz Scheideler; Helga Ninnemann
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 400 KB
- Volume
- 173
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Establishing the relative intracellular proportions of flavins in Neurospora crassa (and in other organisms) in vivo may be hampered by degradation of flavins after homogenization of the cells. The system described here allows separation and identification of intracellular free and bound flavins under conditions restrictive for the FADdegrading enzyme(s). A "protective buffer" containing 0.1 M citrate adjusted to pH 4.0 with K*HPOd, 5 mM ATP, and 0.5 mM EDTA prevents FAD from rapid enzymatic cleavage in crude cell lysates of the Neurospora crassa mutant "slime."