✦ LIBER ✦
Method for evaluating the reliability of distances and rotational correlation times deduced from 2D 1H NMR NOESY experiments
✍ Scribed by D. Genest; J. P. Simorre
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 303 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0749-1581
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✦ Synopsis
Abstract
An experimental method is proposed which selects from all the inter‐proton distances r determined from two‐dimensional NMR NOESY experiments, those which do not depend on the internal motions of the molecule under investigation. Only these distances are quantitatively reliable for modelling purposes, owing to (1/r^6^) averaging and the possible correlation between r and the orientation of two protons as a consequence of the molecular Brownian internal dynamics. All the other distances must be used only qualitatively. Two examples of the application of this method to the lipopeptide stendomycin are given.