Method for electroporation for the early chick embryo
β Scribed by Jun Hatakeyama; Kenji Shimamura
- Book ID
- 108944121
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 132 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0012-1592
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Electroporation has led to new approaches to the analysis of gene regulation of the chick embryonic system. However, application of this method to Xenopus, another model organism of embryology, has left many difficulties to be overcome. The specially devised electrodes, the examination of luciferase
14C-2-deoxyglucose (DC), currently employed in in vivo studies of brain glucose metabolism, has been used for determination of glucose consumption in the in vitro developing chick embryo. DC, presented in traces, accumulates in the embryo in proportion with incubation time. Analysis of tissue homoge