Method for determining location and orientation of macromolecules in membrane-water systems
β Scribed by V. E. Khutorskii
- Book ID
- 112367571
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 525 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0040-5760
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π SIMILAR VOLUMES
A simple and effective method is described for simultaneously measuring dipolar couplings for methine, methylene, and methyl groups in weakly oriented macromolecules. The method is a Jmodulated 3D version of the well-known [ 1 H-13 C] CT-HSQC experiment, from which the J and dipolar information are
It has recently been shown that the degree of alignment of macromolecules in an aqueous dilute liquid crystalline medium of bicelles is sufficient to permit accurate values of residual 15 N-1 H, 13 C-1 H, and 13 C β£ -C dipolar couplings to be obtained on a routine basis, thereby providing potentiall