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Method and use of Recycled [35S]Sulphate in metabolic labelling

✍ Scribed by R. F. Sewell; P. E. C. Brenchley; N. P. Mallick

Publisher
John Wiley and Sons
Year
1989
Tongue
French
Weight
242 KB
Volume
27
Category
Article
ISSN
0022-2135

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✦ Synopsis

Efficiency of [35S]sulphate incorporation into macromolecules in metabolic labelling experiments has been uniformly poor, 0 . 0 0 0 5 -

0. 35% ( 1 -6 ) .

A procedure is described for recovering approximately 90% of the unused [35S]sulphate from such a labelling experiment in a form in which it can be used again, thus improving (asymptotic) incorporation efficiency up to tenfold.

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