Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements

✍ Scribed by Yinduo Ji

• Publisher: Springer US;Humana
• Year: 2020
• Tongue: English
• Leaves: 274
• Series: Methods in Molecular Biology 2069
• Edition: 3rd ed. 2020
• Category: Library

✦ Synopsis

This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens.

✦ Table of Contents

Front Matter ....Pages i-xi
Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections (Elizabeth L. Palavecino)....Pages 1-28
Rapid Methods for Detection of MRSA in Clinical Specimens (Elizabeth L. Palavecino)....Pages 29-45
Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus (MRSA) (Yekaterina Timofeyeva, Ingrid L. Scully, Annaliesa S. Anderson)....Pages 47-58
Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA (Tetsuo Yamaguchi, Daisuke Ono, Ayami Sato)....Pages 59-78
Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains (Yiping He, Sue Reed)....Pages 79-88
Spa Typing of Staphylococcus aureus Isolates (Xin Wang)....Pages 89-94
Multilocus Sequence Typing of Staphylococcus aureus (Ying Liu, Yinduo Ji)....Pages 95-102
Genetic Manipulations of Staphylococcal Chromosomal DNA (Crystal M. Austin, Jeffrey L. Bose)....Pages 103-111
Genetic Manipulation of MRSA Using CRISPR/Cas9 Technology (Weizhong Chen, Quanjiang Ji)....Pages 113-124
Identification of Target Genes Mediated by Two-Component Regulators of Staphylococcus aureus Using RNA-seq Technology (Ting Lei, Junshu Yang, Aaron Becker, Yinduo Ji)....Pages 125-138
Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus (Emilia Bonar, Kinga Chlebicka, Grzegorz Dubin, Benedykt Wladyka)....Pages 139-154
Identification of Virulence Determinants During Host-Pathogen Interaction Using Tn-Seq Technology (Christopher T. Peek, Carolyn B. Ibberson, James E. Cassat)....Pages 155-175
Metabolomic Profiling of Staphylococcus aureus (Ting Lei, Qingqing Mao, Chi Chen, Yinduo Ji)....Pages 177-186
Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells (Nansea Ji, Junshu Yang, Yinduo Ji)....Pages 187-195
Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections Using Noninvasive Optical Imaging (Nathan K. Archer, Yu Wang, Roger V. Ortines, Haiyun Liu, Sabrina J. Nolan, Qi Liu et al.)....Pages 197-228
Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options (Sarah J. Chalmers, Mark E. Wylam)....Pages 229-251
Animal Models for Drug Development for MRSA (Andrea Marra)....Pages 253-266
Back Matter ....Pages 267-268

✦ Subjects

Life Sciences; Bacteriology; Infectious Diseases