## Abstract The methylotrophic yeast __Pichia pastoris__ has been used for the expression of many proteins, including antibody fragments. However, limitations became obvious especially when secreting heterodimeric Fab fragments. Upβtoβdate, antibody fragments have only been expressed under control
β¦ LIBER β¦
Methanol induction optimization for scFv antibody fragment production in Pichia pastoris
β Scribed by A. E. Cunha; J. J. Clemente; R. Gomes; F. Pinto; M. Thomaz; S. Miranda; R. Pinto; D. Moosmayer; P. Donner; M. J. T. Carrondo
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 281 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0006-3592
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An unstructured growth model for the recombinant methylotrophic yeast P. pastoris Mut + expressing the heavy-chain fragment C of botulinum neurotoxin serotype A [BoNT/A(H c )], was successfully established in quasi-steady state fed-batch fermentations with varying cell densities. The model describes