Methanol Elevates Cytosolic Calcium Ions in Cultured Canine Cerebral Vascular Smooth Muscle Cells: Possible Relation to CNS Toxicity
✍ Scribed by Wenyan Li; Tao Zheng; Jun Wang; Bella T. Altura; Burton M. Altura
- Book ID
- 104344355
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 59 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0741-8329
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✦ Synopsis
Acute exposure of cultured canine cerebral vascular smooth muscle cells to methanol (10-400 mM) results in concentration-dependent elevation of the concentration of intracellular free calcium ion ([Ca 2 ϩ ] i ) as measured with the fluorescent indicator, fura-2, and digital imaging microscopy. The resting level of [Ca 2 ϩ ] i in the cerebral vascular smooth muscle cells was 89.3 Ϯ 5.3 nM. Exposure of these cells to 10 mM methanol for only 5 min resulted in significant elevation in [Ca 2 ϩ ] i (i.e., to 105.7 Ϯ 4.6) ( p Ͻ 0.05). Methanol (10 mM) is a concentration found in the blood of victims demonstrating early CNS toxicity. Other, higher concentrations of methanol rapidly raised [Ca 2 ϩ ] i upwards of 60% over basal resting levels. These result suggest that methanol-induced cerebral vasospasm is a consequence of large rises in intracellular Ca 2 ϩ . These events could play a crucial role in methanol-induced cerebral edema, brain hemorrhage, and cerebral and retinal infarcts, eventuating in severe deficits in brain blood flow and the known, subsequent CNS disturbances.