Methanol: A marker of chronic alcohol use

✍ Scribed by B.M. Kapur; Y. Maziar

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 216 KB
• Volume: 26
• Category: Article
• ISSN: 0009-9120
• DOI: 10.1016/0009-9120(93)90045-8

✦ Synopsis

CLINICAL CHEMISTS monolayers grown on microporous membrane inserts for 12 d. RAPA uptake and transport across cell monolayers were compared when 10,000 I~g/L of RAPA (cold) plus 0.05 i~Ci 14C-RAPA was added in combination with varying concentrations (1,000 I~g/L, 10,000 I~g/L and 100,000 ~tg/L) of the following drugs: CsA or RS-61443. Results are shown in the figures above. Increasing concentrations of CsA resulted in a significant dose-dependent decrease in 14C-RAPA transport across cell monolayers. In contrast, at high (100,000 I~g/L) RS-61443 concentrations, ~4C-RAPA transport was significantly increased. Uptake of 14C-RAPA into cell monolayers was significantly decreased only with the 100,000 I~g/L CsA concentration. These studies suggest that combinations of immunosuppressive drugs given orally have a potential for altering the intestinal transport and uptake of RAPA. Additional studies are currently being performed in vitro using multiple combinations of other immunosuppressive drugs and other absorptive intestinal cell lines, as well as in vivo in animals to further investigate this.