Metal oxide coated cell culture arrays for rapid biological screening

✍ Scribed by John D. Jarrell; Tai H. Eun; Marcus Samale; Clyde Briant; Brian W. Sheldon; Jeffrey R. Morgan

• Book ID: 102295374
• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 399 KB
• Volume: 83A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.31446

✦ Synopsis

Abstract

The biointerface of metallic alloy implants is a spontaneously formed metal oxide layer. This study presents a novel method for creating titanium oxide xerogel coated microplates for high‐throughput biological screening that overcomes several limitations of using bulk metal samples to study oxides. Metal–organic precursors were used to evaluate the influence of Al, V, Ca, and P doped smooth and textured titanium oxide xerogel coatings on the bioresponse of human fibroblasts to increase understanding of the soft tissue sealing around transepithelial devices. Coatings made of titanium n‐butoxide were characteristically smooth, while those of titanium isopropoxide were micro‐ and nanofeatured. Screening consisted of WST‐1 proliferation assay, calcein AM cell number and viability assay, and a modified cell seeding efficiency and centrifugation adhesion assay. Small variations in initial attachment and centrifugation adhesion of human fibroblasts were observed among the coatings and comparable to tissue‐culture treated polystyrene. Proliferation and viability at 24 and 48 h were reduced by the 10 and 20% vanadium additions. Metal oxide coated microplates are adaptable to the investigation of a wide range of metal–organic derived chemistries and the influence of oxide texture, and level of oxide crystallinity and oxide grain size on the biological responses of cells. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007