Metal loading and enzymatic degradation of fungal cell walls and chitin

✍ Scribed by C. Krantz-Rülcker; E. Frändberg; J. Schnürer

• Publisher: Springer Netherlands
• Year: 1995
• Tongue: English
• Weight: 549 KB
• Volume: 8
• Category: Article
• ISSN: 1572-8773
• DOI: 10.1007/bf00156152

✦ Synopsis

The capacity of chitin (from crab shells) and of fungal cell walls from Trichoderma harzianum to accumulate zinc, cadmium and mercury was studied as well as the effects of adsorbed metals on the enzymatic hydrolysis by Novozym 234 of the two substrates. The total adsorbing capacity with respect to these metals was estimated to be at least 10 mmol kg -1 chitin (dry weight) and 50 mmol kg -1 fungal cell walls (dry weight), respectively, at pH 6.1. Enzymatic digestion of fungal cell walls preloaded with mercury and cadmium was significantly reduced, while zinc did not cause any significant inhibition. The effect of metal complexation by chitin on the enzymatic digestion was not as pronounced as for fungai cell walls. This could reflect the fact that chitin sorbed a lower total amount of metals. The inhibitory effect of metals on the enzymatic hydrolysis was caused by the association of the metals with the two substrates and not by the presence of free metals in solution.