Metal-Dependent Global Folding and Activity of the 8-17 DNAzyme Studied by Fluorescence Resonance Energy Transfer

Metal-Ion-Dependent Folding of a Uranyl-

Metal-Ion-Dependent Folding of a Uranyl-Specific DNAzyme: Insight into Function from Fluorescence Resonance Energy Transfer Studies

✍ Ying He; Prof. Dr. Yi Lu 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 989 KB

Fluorescence resonance energy transfer (FRET) has been used to study the global folding of an uranyl (UO 2 2 + )-specific 39E DNAzyme in the presence of Mg 2 + , Zn 2 + , Pb 2 + , or UO 2 2 + . At pH 5.5 and physiological ionic strength (100 mm Na + ), two of the three stems in this DNAzyme folded i