Metal-Assisted Hybridization of Oligonucleotides, Evaluation of Circular 2′-O-Me RNA as Ligands for the TAR RNA Target
✍ Scribed by Laurence Zapata; Katell Bathany; Jean-Marie Schmitter; Serge Moreau
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 163 KB
- Volume
- 2003
- Category
- Article
- ISSN
- 1434-193X
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✦ Synopsis
Abstract
Two complementary oligonucleotides were conjugated with terpyridine ligands at their nearby 5′‐ and 3′‐ends. Addition of a stoichiometric amount of a transition metal (Zn^2+^, Fe^2+^) resulted in a large increase in the melting temperature of the duplex. The conjugation of TPY to stem‐loop oligomers provided an efficient procedure for the cyclisation of the oligomer after the addition of metal ions. Such a short stem‐loop oligomer was designed to target the HIV‐1 TAR RNA through loop−loop interactions. The addition of Zn^2+^ ions yielded a good ligand (K~d~ = 30 nM) for this RNA structural element. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2003)