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Metal affinity precipitation of proteins carrying genetically attached polyhistidine affinity tails

✍ Scribed by Gösta LILIUS; Mats PERSSON; Leif BÜLOW; Klaus MOSBACH

Book ID
115128607
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
673 KB
Volume
198
Category
Article
ISSN
1432-1327

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✍ Jonas Carlsson; Klaus Mosbach; Leif Bülow 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 809 KB

Proteins carrying genetically attached polyhistidine tails have been purified using affinity precipitation with metal chelates. DNA fragments encoding four or five histidine residues have been genetically fused to the oligomeric enzymes lactate dehydrogenase (Bacillus stearother- mophilus), 0-glucor