Metagenomics: Perspectives, Methods, and Applications

✍ Scribed by Camilla Benedetti

• Publisher: Nova Publishers
• Year: 2014
• Tongue: English
• Leaves: 205
• Series: Microbiology Research Advances
• Edition: 1.
• Category: Library

✦ Synopsis

Metagenomic analysis has extraordinary potential to improve our understanding of microbial populations in their natural environment and identify novel genes of interest. The key feature of such analyses is that they are performed using metagenomic libraries constructed from total DNA isolated from a particular niche rather than a laboratory culture. Thus, metagenomic analyses potentially allow access to all the genetic resources present in an environment, regardless of whether or not they belong to microorganisms that can be cultured in the laboratory. Sequence-based metagenomic analyses rely.

✦ Table of Contents

METAGENOMICS: METHODS, APPLICATIONS AND PERSPECTIVES......Page 3
METAGENOMICS: METHODS, APPLICATIONS AND PERSPECTIVES......Page 5
Library of Congress Cataloging-in-Publication Data......Page 6
CONTENTS......Page 7
PREFACE......Page 9
ABSTRACT......Page 13
1. MICROBIAL DIVERSITY......Page 14
2. OVERVIEW OF METAGENOMIC LIBRARY CONSTRUCTION......Page 15
3. METAGENOMIC LIBRARY ANALYSES......Page 21
4. FUNCTIONAL SCREENS......Page 24
5. METAGENOMIC GENE EXPRESION......Page 33
6. FUTURE PROSPECTS FOR IDENTIFICATION OF METAGENOMIC FUNCTIONS......Page 40
REFERENCES......Page 41
ABSTRACT......Page 57
2. EXTRACTION OF NUCLEIC ACIDS FROM ENVIRONMENTAL SAMPLES......Page 58
3. ENRICHMENT OF ENVIRONMENTAL SAMPLES......Page 61
4. CONSTRUCTION OF A METAGENOMIC LIBRARY......Page 65
5. SCREENING OF METAGENOMIC DATA......Page 66
REFERENCES......Page 72
ABSTRACT......Page 79
INTRODUCTION......Page 80
REFERENCES......Page 91
1. INTRODUCTION......Page 99
2. EVALUATING ANTHROPOGENIC IMPACTS THROUGH METAGENOMICS......Page 101
3. APPLICATIONS OF METAGENOMICS TO PUBLIC HEALTH......Page 103
REFERENCES......Page 106
ABSTRACT......Page 111
TARGET OF METAGENOMIC ANALYSIS......Page 112
APPROACHES TO METAGENOMIC SCREENING......Page 115
EUKARYOTIC CELLS AS A TARGET RESOURCE FOR METAGENOMIC ANALYSIS......Page 117
EXPLORING THE FUNCTIONAL EUKARYOTIC GENES BY A METATRANSCRIPTOMIC APPROACH......Page 118
CONCLUDING REMARKS AND FUTURE PROSPECTS......Page 119
REFERENCES......Page 120
ABSTRACT......Page 127
INTRODUCTION......Page 128
OVERCOMING THE PROBLEMS IN THE EXTRACTION AND OBTAINING HUMUS-FREE PURE ENVIRONMENTAL DNA......Page 129
PROBLEMS IN SCREENING METAGENOMIC LIBRARIES......Page 130
PROGRESS ACHIEVED IN DISCOVERING A VARIETY OF BIOCATALYSTS......Page 131
OTHER INDUSTRIALLY IMPORTANT ENZYMES......Page 139
CONCLUSION......Page 142
REFERENCES......Page 143
ABSTRACT......Page 149
1. ORIGIN OF METAGENOMICS......Page 150
2. SOIL - THE ECOSYSTEM OF CHOICE FOR METAGENOMIC STUDIES......Page 151
3. ISOLATION OF METAGENOMIC DNA......Page 152
CONCLUSION......Page 162
REFERENCES......Page 163
ABSTRACT......Page 169
LIMITATIONS OF CONVENTIONAL MICROBIAL METHODS......Page 170
THE METAGENOMICS PIPELINE......Page 171
BIOINFORMATICS AND THERMOPHILE METAGENOMICS......Page 174
ANNOTATION OF METAGENOMIC SEQUENCES......Page 175
SEQUENCE-DRIVEN METAGENOMICS......Page 177
FUNCTION-DRIVEN METAGENOMICS......Page 178
HOT WATER SPRINGS......Page 179
THERMOPHILIC MICROORGANISMS IN HOT SPRINGS......Page 180
THERMOPHILIC HOT SPRINGS BACTERIA......Page 181
THERMOPHILIC VIRUSES......Page 182
THERMOPHILIC ARCHEA......Page 183
CONCLUSION......Page 184
REFERENCES......Page 185
INDEX......Page 195