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Metabolomic assays of the concentration and mass isotopomer distribution of gluconeogenic and citric acid cycle intermediates

✍ Scribed by Lili Yang; Takhar Kasumov; Lynn Yu; Kathryn A. Jobbins; France David; Stephen F. Previs; Joanne K. Kelleher; Henri Brunengraber


Publisher
Springer
Year
2006
Tongue
English
Weight
293 KB
Volume
2
Category
Article
ISSN
1573-3882

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The 13C mass isotopomer distribution of liver phosphoenolpyruvate (PEP) yields important information on the regulation of gluconeogenesis and the citric acid cycle. A convenient technique is presented for measuring the mass isotopomer distribution of PEP in tissue extracts. The procedure involves re