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Metabolomic applications of HILIC–LC–MS

✍ Scribed by Simon Cubbon; Carla Antonio; Julie Wilson; Jane Thomas-Oates

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
258 KB
Volume
29
Category
Article
ISSN
0277-7037

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✦ Synopsis

Abstract

Hydrophilic interaction liquid chromatography (HILIC), although not a new technique, has enjoyed a recent renaissance with the introduction of robust and reproducible stationary phases. It is consequently finding application in metabolomics studies, which have traditionally relied on the stability of reversed phases (RPs), since the biofluids analyzed are predominantly aqueous and thus contain many polar analytes. HILIC's retention of those polar compounds and use of solvents readily compatible with mass spectrometry have seen its increasing adoption in studies of complex aqueous metabolomes. This review describes the stationary phases and their features, surveys HILIC–LC–MS's role in metabolomics experiments, discusses approaches to data extraction and analysis including multivariate analysis, and reviews the literature on HILIC–MS applications in metabolomics. © 2009 Wiley Periodicals, Inc., Mass Spec Rev 29:671–684, 2010

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