Metabolites and tissue distribution of resveratrol in the pig

✍ Scribed by María Azorín-Ortuño; María J. Yáñez-Gascón; Fernando Vallejo; Francisco J. Pallarés; Mar Larrosa; Ricardo Lucas; Juan C. Morales; Francisco A. Tomás-Barberán; María T. García-Conesa; Juan C. Espín

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 240 KB
• Volume: 55
• Category: Article
• ISSN: 1613-4125
• DOI: 10.1002/mnfr.201100140

✦ Synopsis

Abstract

Scope: trans‐Resveratrol (RES) and/(or) its metabolites exert many effects in vivo. Our aim was to study the metabolism and tissue distribution of RES using the pig, a mammal physiologically close to humans.

Methods and results: Forty‐seven tissues, organs and fluids were analyzed 6 h after intragastric RES administration (5.9 mg/kg body weight) using HPLC‐MS/MS. Twelve RES and seven dihydroresveratrol (DH‐RES) metabolites were detected. DH‐RES was the main metabolite in cecum, colon and rectum, whereas RES‐3‐O‐glucuronide was the most abundant one in fluids and organs. Approximately 74.5% of the total RES administered was recovered in the form of RES, DH‐RES and derived metabolites (65.1% along the gastrointestinal tract, 7.7% in urine, 1.2% in bile and 0.5% in organs). We report here, for the first time, the occurrence of RES ribosyl‐sulfate derivative, DH‐RES diglucuronide, DH‐RES sulfoglucuronide and DH‐RES disulfate as well as the metabolic profile of RES and DH‐RES in the aorta, lymph, lymph node, ovaries, uterus, cerebellum, pancreas, urinary bladder tissue, fat and muscle.

Conclusion: This study contributes to the clarification of the metabolism and tissue distribution of RES and could help to further understand the mechanisms underlying its effects.