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Metabolism of hela cells: Methodology and media evaluation

✍ Scribed by Gifford, George E. ;Robertson, Hugh E. ;Syverton, Jerome T.

Publisher
Wiley (John Wiley & Sons)
Year
1957
Tongue
English
Weight
554 KB
Volume
49
Category
Article
ISSN
0095-9898

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✦ Synopsis

THREE FIGURES

Strain HeLa cells, derived from an epidermoid cervical carcinoma (Gey, '52; Scherer et al., '53) are currently employed in this laboratory in studies of the physiology and biochemistry of poliovirus replication in animal cells. The use of manometry f o r determination of the metabolitic acivity of HeLa cells has been reported (Gifford et al., '54). This communication relates to the application of manometric procedure t o : (a) estimation of cellular growth, and (b) evaluation of some complex media and buffers for use on HeLa cell cultures.

MATERIALS AND METHODS

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