Metabolism of dextrorphan by CYP2D6 in different recombinantly expressed systems and its implications for the in vitro assessment of dextromethorphan metabolism

A validated SIM GC/MS method for the sim

A validated SIM GC/MS method for the simultaneous determination of dextromethorphan and its metabolites dextrorphan, 3-methoxymorphinan and 3-hydroxymorphinan in biological matrices and its application to in vitro CYP2D6 and CYP3A4 inhibition study

✍ Marios Spanakis; Ioannis S. Vizirianakis; Maria Mironidou-Tzouveleki; Ioannis Ni 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 347 KB 👁 2 views

## Abstract Dextromethorphan is used as a probe drug for assessing CYP2D6 and CYP3A4 activity __in vivo__ and __in vitro__. A SIM GC/MS method without derivatization for the simultaneous determination of dextromethorphan and its metabolites, dextrorphan, 3‐methoxymorphinan and 3‐hydroxymorphinan, i