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Metabolism of [13C1]gibberellin A29to [13C1]gibberellin-catabolite in maturing seeds ofPisum sativumcv. Progress No. 9

✍ Scribed by Valerie M. Sponsel; Jake MacMillan

Publisher: Springer-Verlag
Year: 1980
Tongue: English
Weight: 702 KB
Volume: 150
Category: Article
ISSN: 0032-0935
DOI: 10.1007/bf00385614

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✦ Synopsis

The metabolism of GA29 during seed maturation in Pisum sativurn cv. Progress No. 9 was further investigated. [17-~3C1]GA29 was metabolised to a GA-catabolite (structure 3), with incorporation of the [13C] label from the GA29 substrate into the GAcatabolite being demonstrated by GC-MS. Quantitation of the GA-catabolite using GC-MS was achieved by adding GA-catabolite, labelled with [aso], to seed extracts as an internal standard. At least 50% conversion of [13C~]GA29 to [~3Ct]GA-catabolite was demonstrated with the build up of exogenous [13C1]GA_ catabolite strictly paralleling the accumulation of native GA-catabolite. These results strongly suggest that conversion of GA29 to the GA-catabolite is a natural metabolic step occurring during the final stages of seed maturation. 25 gg per seed of native GA-catabolite was recorded in 37 day old seeds. Some problems encountered in the analysis of extracts containing the GA-catabolite are discussed briefly.

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