I n uiuo "phosphorus nuclear magnetic resonance spectroscopy was uaed to measure the hepatic metabolic state in various groups of rats given ethanol, a control liquid diet or a solid chow diet. The use of selective presaturation pulses applied to the broad phosphorus reaonancea of immobile phospholipids permitted reliable debrmination of ATP/ADP ratios by quantitation of the ATP-/3 and ATP-7 pea% areas. ATP/ADP ratios were d e p d by both techniques in rats chronically ingesting ethanol compared to pair-fed animals consuming the control liquid diet. These differences were observed regardless of whether ethanol feeding was continued up to the time of investigation or whether it was discontinued for 24 hr prior to study. Acute alcohol administration in chow-fed rats, not previously ingesting ethanol, did not lower hepatic ATP/ADP ratios by either methodology. In all case6, liver ATP/ADP ratios 888889ed by *'phosphorus nuclear magnetic resonance spectroscopy were higher than those measured by high-performance liquid chromatography. However, parallel decreases in hepatic ATP/ADP ratios were observed with chronic ethanol consumption by both "phosphorus nuclear magnetic resonance spectroscopy and the biochemical method, confirming the utility of in uiuo slphosphorus nuclear magnetic resonance spectroscopy for aseessment of the hepatic bioenergetic status. The difference in absolute ATP/ADP ratios by the two methods may to some degree be explained by binding effects of ADP with proteins or mitochrondrial membranes, rendering it partially invisible to nuclear magnetic resonance or alternatively, by breakdown of high energy phosphate bonds with freeze clamp extraction.
Biochemical techniques for the assessment of hepatic adenine nucleotide levels are limited by sampling small areas of liver at an isolated time and the propensity for