Metabolic reaction network of Pichia pastoris with glycosylation reactions: Flux analysis for erythropoietin production

Metabolic flux analysis for recombinant

Metabolic flux analysis for recombinant protein production by Pichia pastoris using dual carbon sources: Effects of methanol feeding rate

✍ Eda Çelik; Pınar Çalık; Stephen G. Oliver 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 518 KB

## Abstract The intracellular metabolic fluxes through the central carbon pathways in the bioprocess for recombinant human erythropoietin (rHuEPO) production by __Pichia pastoris__ (Mut^+^) were calculated to investigate the metabolic effects of dual carbon sources (methanol/sorbitol) and the metha